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ATCC Connection Newsletter
Click on the links below to view each newsletter.
Autumn 2007 - Volume 27, Number 2 (http://www.atcc.org/common/documents/pdf/atccv27no2.pdf) Features:- Introducing SafeTsource non-animal origin microbial products which can be used in a wide variety of applications where the presence of animal-derived products may be of concern. The SafeTsource product line included microbial cultures, growth media and a cryoprotectant. They are also applicable for quality control testing where products are manufactured using aseptic processing.
- ATCC have a wide range of clone vector and host sets that are useful for a variety of research projects. This newsletter describes a few of the more popular sets.
- For many experiments it is important to determine the number of live cells and their viability. Among all nonradioactive viability assays, the MTT assay is one of the most versatile and trusted options. The ATCC MTT Cell Proliferation Assay Kit provides an easy to use tool for studying the induction and inhibition of cell proliferation in any in vitro model.
- Introducing Authenticult, ATCC genuine cultures in new packaging. Authenticult provides quality control testing laboratories with convenient, easy to use, cost-effective, ATCC Genuine Cultures in a self-contained package that includes lyophilised microorganism pellet, reservoir of hydrating fluid and inoculating swab.
- New Genomic DNA from fully sequenced strains – laboratories around the world have sequenced the genomes of a wide range of microbes. New genomic DNA from full sequenced, well-characterised microbial strains are now available in 5ug package sizes. ATCC testing has expanded to include the determination of the total amount of DNA by PicoGreen measurement.
- ATCC have introduced new hTERT-immortalised cell lines.
- ATCC and Roche Applied Science have combined resources to benefit life science investigators by consolidating information on transfection and routinely used cell lines. The partnership will provide efficiencies in experimental design and can improve the rate of successful transfection for the life science research community.
- An article describing the effects of passage number on cell line transfection. An important factor in the success of any transfection experiment is the quality of the cells and the passage number of the cell lines can affect not only transfection efficiency but protein expression as well.
Features:
- Cryongenic preservation of bacteria - an informative article describing the factors that are critical to the stability and viability of a bacterial culture undergoing cryopreservation. The article discusses growth and preparation for cryopreservation, cryoprotectants, cooling and storage conditions and recovery of frozen cultures.
- ATCC have recently launched an easy detection assay (ELF Phospatase Detection Kit) for determining whether embryonic stem cells have maintained their undifferentiated state or if they have begun to differentiate.
- ATCC scientists have identified a list of acquisition target categories that have the highest utility for the widest range of research fields. In addition to respiratory pathogens and disease models, ATCC now seeks hybridomas, micro-organisms with fully sequenced genomes and immune effector cells.
Features:
- ATCC and amaxa GmbH have entered into an agreement whereby ATCC will supply amaxa with cell lines to develop and optimize protocols using amaxa’s Nucleofector technology. The primary goal of the collaboration is to enable researchers to work with optimized and ready-to-use nonviral transfection protocols on a wide range of cell lines.
- The Oregon Collection of Methanogens (OCM) is in the process of being transferred to ATCC. The OCM is the premier collection of methanogenic archaea in the world, containing novel methanogens curated by the late Dr David Boone of Portland State University.
- Mouse transcription factor clones from the Dana-Farber Cancer Institute and GENSAT-BAC clones from Rockefeller University and NINDS are now searchable online.
Features:
- Longer life to cells in culture with TERT immortalization products.
- Use of repetitive sequence based PCR for genotyping archaea.
Features:
- Replicated DNA Clone Plates - If your genomics research involves high throughput screening methods, purchasing entire plates of clones or clone sets may be the fastest and most cost-effective way to achieve results. ATCC has full-length. Putative full-length and EST plates from the IMAGE Consortium as well as other sets and collections. Read more about these sets and review the complete list of plates that are currently available.
- Stem Cell Pluripotency - Pluripotent stem cells, including embryonic stem cells, embryonal carcinoma cells and embryonic germ cells exhibit unique properties that have been exploited for stem cell derivation, purification and analysis. A group of researchers at the Jackson Laboratory and the Stem Cell Center at ATCC report on a dual-colour assay that was used to assess overall in vitro embryonic stem cell pluripotency.
- Cryogenic Preservation of Animal Cells - a highly informative article describing how the cryopreservation of cell cultures can be optimized. This article provides information on the freezing medium, the equipment used to freeze cells, the methods of freezing and how to optimally recover cryopreserved cells.
Features:
- Microbial DNA - ATCC has purified genomic DNA from over 200 microbial strains, suitable for use in PCR, Southern blotting and library construction.
- Bacterial Two-Hybrid System - ATCC’s bacterial two-hybrid system contains two E. Coli hosts and six expression vectors. It is useful for studying bacterial protein-protein interactions in vivo.